Protein Purification Reagents and Kits
STEMCELL Technologies EasySep™ Human CD138 Positive Selection Kit II
The EasySep™ Human CD138 Positive Selection Kit II is designed to isolate CD138+ (syndecan-1) cells from fresh or previously frozen bone marrow or peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD138 and dextran-coated magnetic particles. This cocktail also contains an antibody to human Fc receptor to minimize nonspecific binding. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off. The CD138 antigen is expressed on normal and malignant plasma cells (but not mature B cells).This product is an improved version of the EasySep™ Human CD138 Positive Selection Kit (Catalog #18357) that provides highly purified cells using a shorter protocol.
STEMCELL Technologies EasySep™ Rat CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Rat CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
STEMCELL Technologies RoboSep™ Human CD8+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Human CD8+ T Cell Isolation Kit is designed to isolate CD8+ T cells from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube. This product can be used in place of the EasySep™ Human CD8+ T Cell Enrichment Kit (Catalog #19053) for even faster cell isolations.
Biotium CD45RA (Leukocyte Marker)(K4B5), CF740 conjugate, 0.1mg/mL
Recognizes a protein of 205 kDa-220 kDa, identified as CD45RA. CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4 T-cells, 50% of peripheral CD8 T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4 or CD8 T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO ve) from B cell lymphomas (CD45RA ve). Primary antibodies are available purified, or with a selection of fluorescent CF Dyes and other l
STEMCELL Technologies RoboSep™ Human Neutrophil Isolation Kit, For processing: 100 mL whole blood processed
The EasySep™ Direct Human Neutrophil Isolation Kit isolates functional, highly purified neutrophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.This product replaces the EasySep™ Human Neutrophil Isolation Kit (Catalog #19257) for even faster cell isolations.
STEMCELL Technologies RoboSep™ Mouse CD19 Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD19 Positive Selection Kit II is designed to isolate CD19+ cells from single-cell suspensions of splenocytes or other tissues by positive selection. Desired cells are targeted with antibody complexes recognizing CD19 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.This product replaces the EasySep™ Mouse CD19 Positive Selection Kit (Catalog #18754) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
Invent Biotechnologies Inc Minute Single Nucleus Isolation Kit for Tissues/Cells, Catalog Number SN-047, Processing Time ~30 min, Minimum Tissue Input 1 mg, Recommended Tissue Input 15-20 mg, Minimum Cell Input 200,000 cells, Recommended Cell Input 4-5 million cells, 20 preps
The Minute Single Nucleus Isolation Kit is designed for the efficient isolation and purification of single nuclei from various tissue and cell types. This kit overcomes common challenges in traditional nuclei isolation, such as aggregation and doublet formation, by providing a simple, rapid, and highly effective protocol. It is ideal for applications that require high-quality, well-separated single nuclei, including FACS analysis, single nucleus RNA-seq, immunofluorescence staining, and cell cycle analysis.
- Enhanced single nuclei yield to minimize aggregation and doublet formation.
- Rapid processing time of approximately 30 minutes for nuclei isolation.
- Broad compatibility with fresh or frozen tissues and cultured cells.
- Low input requirement that works with 1-30 mg of tissue.
- Suitable for diverse downstream applications such as RNA-seq and ATAC-seq.
STEMCELL Technologies EasySep™ Human Pan-ILC Enrichment Kit, For processing: 1 x 10^9 cells
The EasySep™ Human Pan-ILC Enrichment Kit is designed to enrich Group 1, 2, and 3 Innate Lymphoid Cells (ILC1, 2, and 3) from leukapheresis products by immunomagnetic negative selection. Unwanted cells are targeted for removal by Tetrameric Antibody Complexes directed against non-ILCs and dextran-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as enriched ILCs are simply poured off into a new tube. Isolated cells are immediately available for flow sorting and other downstream applications.
Genscript Corporation CytoSinct 1000 Cell Isolation
GenScript has developed the CytoSinct 1000 Cell Isolation system for easy automated isolation or depletion of human cells Scientists can now isolate up to 120x10 9 total cells (full leukopak) in a single run within 2 hours
STEMCELL Technologies EasySep™ Mouse CD11c Positive Selection Kit II, For processing: 2 x 10^9 cells
The EasySep™ Mouse CD11c Positive Selection Kit II isolates highly purified CD11c+ cells from splenocytes or other tissues by immunomagnetic positive selection. Desired cells are targeted with antibodies and magnetic particles, and isolated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately ready for downstream applications such as flow cytometry, culture, and cell-based experiments.This product replaces the EasySep™ Mouse CD11c Positive Selection Kit (Catalog #18758) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
STEMCELL Technologies RoboSep Release Mouse CD138 Po, 2 x 10.9 cells
Immunomagnetic positive selection of particle-free mouse CD138 cells
STEMCELL Technologies EasySep™ Mouse Memory CD4+ T Cell Isolation Kit, For processing: 1 x 10^9 cells
The EasySep™ Mouse Memory CD4+ T Cell Isolation Kit is designed to isolate pan-memory CD4+ T cells from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-memory CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD45R, CD45RB, CD49b, CD105 and TCRγ/δ) and streptavidin-coated magnetic particles (RapidSpheres™ ). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.Learn more about our next-generation EasySep™ mouse cell isolation kits (/easysep-15-minute-kits), featuring RapidSphere™ technology.
American Research Products Inc Mouse eosinophil cationic prot
Mouse eosinophil cationic protein ECP ELISA Kit from CUSABIO Detection Range 0 156 ng/mL-10 ng/mL Sensitivity 0 039 ng/mL Sample serum plasma cell culture supernates tissue homogenates Method Sandwich
STEMCELL Technologies EasySep Rat CD8 T Cell Isolati, 1 x 10.9 cells
Immunomagnetic isolation of untouched rat CD8 T cells
Genscript Corporation CytoSinct CD4 Nanobeads huma
The CytoSinct CD4 Nanobeads human (GMP) is used for in vitro isolation or depletion CD4 cells from fresh or frozen peripheral blood mononuclear cells (PBMCs) Ieukapheresis products or single cell suspension based on the surface expression of human CD4 Due to the nanoscale structure of the CytoSinct CD4 Nanobeads human (GMP) the excess reagent can be removed by centrifugation or conventional supernatant replacement